Journal: PLOS ONE

Article Title: CDCP1 (CUB domain containing protein 1) is a potential urine-based biomarker in the diagnosis of low-grade urothelial carcinoma

doi: 10.1371/journal.pone.0281873

Figure Lengend Snippet: (A) Immunohistochemical staining with antibody against CDCP1 was performed to evaluate CDCP1 expression on the tissue arrays of UC. IHC tissues were observed and photographed at 40X magnification with an optical microscope (Olympus, Tokyo, Japan). Images were representative of negative, weak, moderate and strong CDCP1 staining at specimens of the normal, low grade, and high grade. (B) The intensity of CDCP1 expression was correlated with tumor grade. (C) The staining percentage of CDCP1 was correlated with tumor grade. (D) The product of the intensity and staining percentage of CDCP1 was correlated with tumor grade. Scale bar indicates 50 μm. Statistical analyses were evaluated by one-way ANOVA with Tukey’s multiple comparisons test. The results were presented as mean ± SD (normal n = 16; low grade n = 76; high grade n = 57, * p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: For IHC of CDCP1, paraffin-embedded human bladder tissue microarray slides were purchased from Biomax (BLC1501, BL601a, and BL208, Biomax Inc., Rockville, MD, USA).

Techniques: Immunohistochemical staining, Staining, Expressing, Microscopy

Journal: PLOS ONE

Article Title: CDCP1 (CUB domain containing protein 1) is a potential urine-based biomarker in the diagnosis of low-grade urothelial carcinoma

doi: 10.1371/journal.pone.0281873

Figure Lengend Snippet: Urine-based cells of UC patients were collected from NCKUH. CDCP1 ICC was performed by using its specific antibody as described in “Material and methods”. (A) Increased CDCP1 was observed in UC specimen. (B) The CDCP1 ICC images of three UC patients were presented. The criteria to evaluate CDCP1 expression were indicated as negative (ICC intensity = 0), weak (ICC intensity = 1), moderate (ICC intensity = 2) and strong (ICC intensity = 3). Images of tissue specimen and urine cytology were taken at 400x magnification. Scale bar indicates 50 μm.

Article Snippet: For IHC of CDCP1, paraffin-embedded human bladder tissue microarray slides were purchased from Biomax (BLC1501, BL601a, and BL208, Biomax Inc., Rockville, MD, USA).

Techniques: Expressing

Journal: PLOS ONE

Article Title: CDCP1 (CUB domain containing protein 1) is a potential urine-based biomarker in the diagnosis of low-grade urothelial carcinoma

doi: 10.1371/journal.pone.0281873

Figure Lengend Snippet: Cell lysates were collected from (A) 5637 and T24 cells or from (B) parental and 5637-CD cells to perform Western blot for detecting CDCP1, n-cadherin, e-cadherin, MMP2, and β -actin. (C) Parental and 5637-CD cells were harvested to seed on the upper chamber of Transwell ® , then the abilities of migration were measured as described in the “Materials and methods” section. Images of migration were taken at 200x magnification. Scale bar indicates 100 μm. In addition, T24 cells were transfected the CDCP1 specific shRNA plasmids for 24 hrs, and then harvested for the detection of (D) mRNA level, or (E) protein level of EMT markers, respectively. (F) Besides, the migration abilities of CDCP1-silenced T24 cells and parental cells were measured as described in “Materials and methods” section. Images of migration were captured at 400x magnification. Scale bar indicates 50 μm. Statistical analysis were analyzed by unpaired two-tailed Student’s t-test. The results were presented as mean ± SD (n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: For IHC of CDCP1, paraffin-embedded human bladder tissue microarray slides were purchased from Biomax (BLC1501, BL601a, and BL208, Biomax Inc., Rockville, MD, USA).

Techniques: Western Blot, Migration, Transfection, shRNA, Two Tailed Test

Journal: PLOS ONE

Article Title: CDCP1 (CUB domain containing protein 1) is a potential urine-based biomarker in the diagnosis of low-grade urothelial carcinoma

doi: 10.1371/journal.pone.0281873

Figure Lengend Snippet: Urine-based cytology is non-invasive and widely used for clinical diagnosis of UC, but its sensitivity is less than 40% for low-grade UC detection. We found a new biomarker CDCP1, which plays a critical role in the progression of UC and significantly increases in low-grade UC. We suggest CDCP1 may have potential as a urine-based biomarker for detecting low-grade UC in urinary cytology.

Article Snippet: For IHC of CDCP1, paraffin-embedded human bladder tissue microarray slides were purchased from Biomax (BLC1501, BL601a, and BL208, Biomax Inc., Rockville, MD, USA).

Techniques: Biomarker Discovery